1) What is the primary mechanism of action for steam sterilization (autoclaving)? a) Oxidizing cellular components b) Breaking microbial DNA strands c) Coagulating and denaturing proteins d) Generating free radicals to eradicate microbes 2) Which sterilization method is considered the best choice for heat-sensitive, single-use devices like catheters and stents due to its excellent penetration? a) Dry Heat sterilization b) Hydrogen peroxide plasma c) steam sterilization d) Ethylene oxide gas 3) Biological Indicators are considered the "gold standard" for validating sterilization. What is the definitive sign of a successful sterilization cycle when using them? a) a chemical indicator changes colour b) the biological indicators spores show no growth after incubation c) the packaging of items remains intact d) the biological indicator spores show how rapid growth after incubation 4) A surgeon needs to sterilize a delicate fiber-optic endoscope. Which method is most appropriate, as it is effective at low temperatures and leaves no toxic residue? a) Dry heat sterilization b) radiation sterilization c) steam sterilization d) hydrogen peroxide plasma 5) What is the key limitation of filtration as sterilisation method? a) it leaves toxic residues on equipment b) it is unsuitable for heat sensitive liquids c) it does not remove viruses until ultra filters are used d) it requires very long processing time

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