1) Which is the MOST important characteristic of an enzyme? a) It is amphoteric b) It has a specific three-dimensional shape c) It is sensitive to changes in the temperature d) It is sensitive to changes in the pH 2) Which of the following statements regarding enzyme is TRUE? a) Enzyme decreases the free energy change of a reaction b) Enzyme changes the direction of chemical reaction c) Enzyme increases rate of reaction d) Enzyme prevents changes in substrate concentration 3) An enzyme catalyses a reaction by … a) lowering the energy of activation of a reaction. b) supplying the energy to speed up a reaction. c) lowering the free energy of a reaction. d) changing the equilibrium of a spontaneous reaction. 4) Increasing the substrate concentration in an enzymatic reaction could overcome which of the following? a) Denaturation of the enzyme b) Allosteric inhibition c) Competitive inhibition d) Saturation of enzyme activity 5) According to the induced fit hypothesis of enzyme catalysis, which of the following is a) The binding of the substrate changes the shape of the enzyme’s active site b) The binding of the substrate depends on the shape of the active site c) Some enzymes change their structure when activators bind to the enzyme d) A competitive inhibitor can outcompete the substrate for the active site

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