Sterilization - Provides high speed of fermentation and high quality of the target product, Inoculum preparation - Provides high velocity of fermentation, reduction of lag-phase time, Fermentation - Central part of biotechnological process, Medium design - Provides the producer strain with all necessary nutrients, Bioreactor - Closed vessel for fermentation, Primary metabolites - Are formed in logarythmic phase and derive from central metabolic pathways, Secondary metabolites - Are formed during stationary phase, are not crucial for microorganism physiology,

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