Diabetes is a chronic disease that creates issues with, how the body metabolizes blood sugar, reading and writing skills, risks of diabetes includes, but is not limited to, circulatory system, nervous system, immune system, possible death, sickle cell anemia, in 1909, what term was coined, insulin, hemoglobin, Nicolas Paulescu worked with what diabetic animal, dog, cat, who was the first person to be injected with pancreatic fluid from cows, Leonard Thompson, George Walden, Historically, what animal's insulin was extracted for human use, pig, bird, true or false: insulin is a single protein, true, false, what issue were reseachers trying to fix in the 1930's, insulin had a short duration period, insulin had too long of a duration, H.C. Hagedorn, Scott, and Fisher had developmental approaches for enhanced insulin. What was the combined approach called, neutral protamine Hagedorn, heterodimeric peptide, Advancements with chromatography led to what in the 1970's, 90% purity, 50% purity, what is one reason why researchers began looking into genetic engineering, patients were having allergic reactions to insulin extracted from animals, animals were producing too much insulin, how is recombinant DNA made, by inserting synthetic genes into plasmids at promoter sites, then into bacteria, so that combined chains make active insulin, by pigs and cattle, recombinant DNA was used to produce insulin from, bacteria, pigs, true or false: physiological factors play a role in how the body responds to insulin drugs, false, true

Genetic Engineering

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