petri dish and agar serilised - to kill unwanted bacteria, innoculating loop passed through a flame - to sterilise it / kill unwanted bacteria, innoculating loop used - to spread bacterium onto agar, lid of petri dish opened as little as possible - prevent microbes from the air entering, petri dish sealed with tape - prevent microbes form the air entering, petri dish incubated - to allow growth of bacteria, petri dish incubated at 250C - so no human pathogens grow,

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