Formation of new species from the pre-existing species// through evolution., Allopatric (speciation), Sympatric (speciation), i) Gene flow is interrupted/reduced due to geographical barrier, ii) A population forms a new species while geographically isolated, iii) Population undergo evolutionary change, iv) Reproductive isolation established, Isolation/Genetic drift/Hybridisation/Adaptive radiation, Habitat Isolation/Temporal Isolation/Behavioral Isolation/Mechanical Isolation/Genetic Isolation, Doubling the number of chromosome (to make hybrid fertile)/ chromosome doubling/overcome hybrid sterility.

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