1) A microscope has a 4x ocular lens and a 10x objective, what is this microscope's total magnification? a) 4x b) 14x c) 40x d) 400x 2) To focus a specimen, it is best to start with which objective a) high power b) low power c) scanning d) ocular 3) What are the three parameters required for successful steam sterilization to occur? a) Temperature, pressure, and time b) Heat, UV-C radiation, and time c) Bleach, ammonia, and water 4) During the sterilization phase, an autoclave chamber typically operates at a pressure of __ above atmospheric pressure a) 15psig b) 8psig c) 45psig 5) During sterilization, a liquid load is most often heated to a temperature of __ a) 250°F b) 212°F c) 260°F 6) Some of the differences between a fume hood and a biological safety cabinet (BSC) are that a BSC protects both the user and the material inside the cabinet and that the exhaust is HEPA filtered. a) True b) False 7) Which of the following practices should be utilized when working in a biological safety cabinet? a) Disinfect the work surface of the BSC before and after work  b)  Disinfect all items which go into and come out of the BSC c) Do not store any items in the BSC d) Allow the BSC to operate before work begins and after work ceases e) All of the above 8) Which of the following definitions best describes disinfection? a) The use of antimicrobial agents on inanimate objects to destroy all non-spore-forming organisms that could pose a hazard to humans or compromise the integrity of an experiment b) Processing clean items to kill all living agent c) Application of a liquid antimicrobial chemical to living tissue to prevent infection d) Heat treatment of a liquid for the destruction of non-sporeforming organisms 9) What is the major advantage of steam sterilization over dry heat sterilization? a) It can be used for the sterilization of anhydrous materials. b) It kills a greater spectrum of organisms. c) It is not as destructive to materials. d) It sterilizes in less time. 10) The dispensing of uninoculated primary rhesus monkey kidney tissue culture into sterile culture tubes is most appropriately conducted in which of the engineering devices listed below? a) Class I biological safety cabinet b) Class II biological safety cabinet c) Vertical laminar flow clean bench d) Horizontal laminar flow clean bench 11) Which of the following media is most enriched? a) Chocolate agar b) Nutrient agar c) Blood agar 12) All of the following are true about agar except it a) liquefies at 100°C. b) is a polysaccharide derived from a red alga. c) Solidifies at approximately 40°C. d) is metabolized by many bacteria. e) facilitates obtaining pure cultures. 13) Which of these media, if any are chemically defined? a) A b) B c) A and B d) A and C e) None of these 14) The addition of which of the following would change a chemically defined medium into a complex medium? a) Biotin (a vitamin) b) K2HPO4 c) NH4NO3 d) Maltose e) Yeast extract 15) Which of the following procedures can be used to isolate a pure culture of a bacterium from a mixture? a) streak plating b) dilution plating c) enrichment culture d) All the above can be used to isolate a pure culture of a bacterium from a mixture. e) None of the above can be used to isolate a pure culture of a bacterium from a mixture. 16) Addition of blood to a culture medium only allows the hemolytic bacteria that grow on the plate to be picked out. This is an example of a a) Differential media. b) Liquid media. c) Chemically defined media. d) Selective media. e) Complex media. 17) You have to pipette 4 microliters of your isolated DNA to 46 microliters of the PCR Master Mix. Which micropipette would you use for to pipette 4 microliters? a) P2 b) P20 c) P200 d) P1000 18) If you are using a P20, and the micropipette numbers are "1", "5", and "0" (in that order), how many microliters are you pipetting? a) 0.15 microliters b) 1.5 microliters c) 15.0 microliters d) 150 microliters 19) A great way to avoid cross-contamination of sample would be to use a new pipette tip every single time you pipette. A new pipette tip can be added, used, and ejected without ever touching the pipette tip directly with your hands a) True b) False 20) At what point do you press the micropipette PAST the stop button? a) When you want to take the liquid into the micropipette. b) When you want to eject the pipette tip c) When you want to dispense the liquid out of the micropipette. d) All of the answers here are correct.

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