DNA helicase - Catalyze unwind the double stranded DNA to become single stranded., Single-strand binding protein (SSB Protein) - To stabilize and prevent the single stranded DNA from recoiling in helix until the strands are replicated., Topoisomerase - Helps relieve the strain caused by the unwinding of DNA double stranded., Primase - Synthesize RNA primer by adding RNA nucleotide that complementary to the DNA template., DNA polymerase III - Add free DNA nucleotides to the 3’ end of the short RNA primer.,

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