Target DNA - segment of DNA that has high commercial value that is beneficial to human, Restriction enzyme - endonuclease enzyme which recognize a specific DNA sequence and cut at a specific point with the restriction site , DNA cloning vector - DNA molecule that can carry foreign DNA fragment into a host cell, Host cell - organism that act as the living host for replication of recombinant DNA, Modifying enzyme - can be used to alter the structure of DNA in vitro,

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