1) The product of reverse transcription is called... a) mRNA b) RNA c) DNA d) cDNA 2) The template for reverse transcription is found in the a) Nucleus b) Cytoplasm c) DNA d) cDNA 3) This enzymes joins sticky ends together a) Polymerase b) Helicase c) Reverse transcriptase d) Ligase 4) This enzyme breaks hydrogen bonds a) Polymerase b) Ligase c) Restriction endonuclease d) Helicase 5) This enzyme is responsible for complementary base pairing a) helicase b) polymerase c) restriction endonuclease d) ligase 6) Restriction endonucleases digest at a) Codons b) Primers c) Antiparallel bases d) Sticky ends 7) Digestion of DNA to reveal sticky ends is a _________ reaction a) hydrolysis b) condensation 8) This means to bind sticky ends to another piece of DNA a) Bond b) Ligate c) Anneal 9) DNA unzips a) at 72oC b) at 95oC c) at 55oC d) With helicase e) Option 2&4 10) A short piece of DNA with bases which are complementary to the bases at the start of a DNA fragment is called a) A DNA probe b) A Ligate c) A codon d) A primer 11) How many copies of a gene would result from 5 PCR cycles? a) 5 b) 32 c) 64 d) 128 12) DNA fingerprinting compares a) Coding DNA b) Non coding DNA 13) To amplify the results of the electrophoresis. the DNA sample is subjected to ________ before separation a) Heat treatment b) UV light c) Xray photography d) PCR 14) The buffer solution used in electrophoresis a) Maintains pH b) Conducts electricity 15) If two bands are produced for locus 1 and one band is produced for locus 2 then... a) The person has homozygous 1 and heterozygous 2 b) The person has heterozygous 1 and homozygous 2 c) The person has genes d) The person has alleles 16) A gene can be located using a) Restriction endonucleases b) Gene sequencing c) Isolation d) A gene probe 17) In a microarray, the person's DNA fragments are found in a) The well b) The wash over solution 18) In gene probing, what has a tag on it a) The person's DNA fragments b) The gene probe c) Both 19) Why is gene probing useful to a genetic counsellor a) They can tell the patient that they don't have the disease b) They can tell the person that they do have the disease c) They can advise the patient of treatment options d) They can advise the patient to encourage other family members to be tested e) They can advise both option three and four 20) A gene probeis a) An allele which matches a gene b) An allele which is complemetary to a gene c) A piece of DNA which matches an allele d) A piece of DNA which is complementary to an allele

Quiz on everything up to restriction mapping

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