Extract chromosome with gene for human insulin from nucleus of a human cell, Cut the gene for human insulin from the chromosome using a RESTRICTION ENZYME, Cut open a bacterial plasmid (vector) using the SAME RESTRICTION ENZYME, Join the human DNA and plasmid USING DNA LIGASE ENZYME, The vector (RECOMBINANT PLASMID) is inserted into a bacterial cell e.g. E.Coli, The bacteria is grown in a fermenter, producing human insulin which is collected and purified for use by diabetics. .

Genetic engineering - producing human insulin (iGCSE Edexcel focus terminology)

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