What are the two phases of chromatography, The mobile phase and stationary phase, What is the mobile phase?, The solvent that moves through the paper, carrying different substances with it, What is the stationary phase?, The chromatography paper, Example of an aqueous solvent , Water - used as a solvent, Example of a non-aqueous solvent , Alcohol - used as a solvent, Pure substances produce how many spots?, Only one spot, Impure substances produce how many spots?, More than one spot, Two substances are likely to be the same if ..., they produce the same number of spots, and these match in colour and position, What is the formula for Rf value?, Distance moved by a spot / distance moved by the solvent front, If a carton reads "pure orange juice" does that mean it is a pure substance?, No - it contains different substances mixed together, a pure substance consists only of ..., only one element, What does a mixture consists of?, Two or more substances not chemically joined together, What does Rf stand for?, Retention factor, What is the affinity of the substance being tested?? , How well it 'sticks' to the chromatography paper, The most soluble substance being tested will ..., Moves furthest up the chromatography paper..

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