1) What is the ideal CFU? a) 30-300 b) 10-30 c) 250-500 d) None of the above 2) Which method is most used by prokaryotes to reproduce? a) Meiosis b) Binary fission c) Mitosis d) Photosynthesis 3) If the solution is __________, this means that there is ZERO net movement- there is an equal amount of water moving in and out of the cell a) Hypotonic b) Isotonic c) Hypertonic d) Myotonic 4) Which type of bacteria does not require oxygen but will grow better in the presence of it? When I’m a tube of thioglycolate, where microbes are more concentrated? a) Obligate aerobes b) Aerotolerant anaerobes c) Microaerophiles d) Facultative Aerobes/anaerobes 5) MacConkey Agar is an example of what type of media? a) Selective and differential media b) Differential media c) Nonselective media  6) All human pathogens are  a) Psychrophiles  b) Mesophiles c) Thermophiles  7) What temperature range is considered “the Danger zone” promoting pathogenic microbial growth?  a) 30 - 130 F b) 20 - 120 F c) 40 - 140 F d) 10 - 100 F 8) In which growth phase will there be an equal rate Of cell division and cell death? a) Lag phase b) Log phase c) Stationary phase d) Death/decline phase W 9) The streak method is used to isolate colonies by diluting cells and sterilizing per quadrant streak? a) True b) false 10) Total volume of sterile media = Total volume of dilutions — total volume of inoculums.  a) True b) False 11) There are both direct and indirect methods of measuring microbes, under which category does testing the turbitidy of a solution fall under? a) Direct b) Indirect 12) Microorganisms that grow best at a PH between 8.0 and 10.5 are called: a) neutrophiles b) mesophiles c) acidophiles d) alkaliphiles 13) If your original population contains 10 cells, and your cells have a generation of 30 min, how many cells will you have after 3 hours? a) 190 cells b) 640 cells c) 300 cells d) 920 cells  14) your original culture media has roughly 20,000 CFUs/ML and you have a total dilution volume of 10mL each. Each dilution factor is 10, how much sterile media will be needed to have a final dilution of 500 CFUs/mL? a) 11.1 mL b) 14.4 mL c) 15.0mL 15) agar remains liquid at >80 degrees celcius, and solid <40 degrees celcius. a) true b) false

chapter 9

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