1) Lac operon is an example of a) only positive regulation b) only negative regulation c) both positive and negative regulation d) sometimes positive sometimes negative 2) In the presence of lactose, how long does it take for the lac operon to be expressed? a) when lactose equals glucose concentration b) when glucose is more than lactose concentration c) as long as lactose is more than glucose concentration d) as long as lactose is more than galactose concentration 3) Which of these acts as an inducer of the lac operon? a) Allolactose b) Lactose c) Galactose d) Glucose 4) The sequence of the structural genes in the lac operon is a) lacA-lacZ-lacY b) lacZ-lacY-lacA c) lacZ-lacA-lacY d) lacA-lacY-lacZ 5) Regulation of the lac operon can be envisioned as regulation of enzyme synthesis by its a) lactose b) substrate c) carbohydrates d) all of the above 6) Lac Operon will be turned on when a) Lactose is less than glucose b) Lactose is less in the medium c) Lactose is more than glucose d) Glucose is enough in the medium 7) In Lac-operon, the gene product of LacA gene is a) Beta-galactoside permease b) Beta-galactoside transacetylase c) Beta-galactosidase d) Beta-galactoside isomerase 8) This condition in lac operon facilitates the condition of lac genes being transcribed at high levels a) low glucose, high lactose b) low glucose, low lactose c) high glucose, high lactose d) high glucose, low lactose 9) The correct option regarding the lac operon in E.coli from the following is a) Lac operon is switched on in the absence of lactose b) Lac repressor binds to the lac promoter c) β-galactosidase is the only enzyme produced in large quantities when lac operon is turned on d) lac operon messenger RNA is a polycistronic mRNA 10) Which of the following events occurs at a Lac operon when lactose levels are high and glucose levels are low? a) cAMP levels decrease, triggering binding of CAP to RNA polymerase b) cAMP activates CAP, which binds to the Lac promoter c) cAMP activates CAP, which binds to the Lac repressor protein d) CAP binds to the ribosome to prevent translation

Tutorial 7

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