Optimum pH for enzyme, Optimum temperature for enzyme, Enzyme & substrate particles move slowly (less kinetic energy). Lower rate of collisions (collisions are less frequent). Fewer successful collisions in a given period of time., The enzyme (made from protein) has been denatured by heat. The shape of the active site has changed so the substrate no longer fits into the active site (the shapes are not complementary)., The enzyme has been denatured by the low pH (possibly acidic). The shape of the active site has changed., The enzyme has been denatured by the high pH (possibly alkaline). The shape of the active site has changed..

Effect of temperature and pH on enzyme activity

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