The flame creates a convection current above the bench, preventing contamination from any microorganisms in the air., The Petri dish and culture medium are heated to a high temperature to kill any potential microorganisms that could contaminate the experiment., Any microorganisms on the loop are killed to prevent contamination., This decreases the risk of microorganisms contaminating the dish., This prevents drops of condensation from dropping onto the surface of the agar and diluting the colony. The tape prevents entry of pathogens but still allows entry of oxygen for aerobic respiration., This restricts the growth of harmful pathogens, which are more likely to grow at higher temperatures.

Aseptic technique

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