1) What are the two single-molecule localization methods in super-resolution fluorescence techniques ? a) STORM and STED b) PALM and STORM c) SIM AND STED d) ME and YOU 2) What are the two patterned illumination methods in super-resolution fluorescence techniques? a) STORM and STED b) PALM and STORM c) SIM AND STED d) SIM AND STORM 3) What is the shape of the depletion laser of the STED beam? a) torus b) circle c) sphere d) cylinder 4) What super-resolution technique uses a second beam to switch off molecules? a) SIM b) PALM c) STORM d) STED 5) What is the reason why the whole process in SIM is repeated through a series of angles using a grid pattern? a) To obtain an uneven enhancement in all directions b) To obtain an equivalent enhancement in all directions c) both b and d d) To have fun 6) SIM can only improve the resolution by a factor of _______? a) 6 b) 4 c) 3 d) 2 7) What is a super-resolution technique that uses a grating pattern of light to illuminate the sample? a) SIM b) STED c) STORM d) PALM 8) What do you call an interference pattern created by overlaying two grids with different angles or mesh sizes? a) Moore pattern b) Moiré pattern c) Moiiré pattern d) None of the above 9) All are super-resolution fluorescence methods, EXCEPT? a) SIM b) STEAD c) STORM d) PALM 10) All are super-resolution methods, EXCEPT. a) Structured illumination microscopy b) Stimulated emission depletion microscopy c) Photoactivated Localization Microscopy d) Stochastic Optical Reconstruction Microscopy e) None of the above

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