Add 20cm3 of milk to the 100cm3 beaker, Add 5cm3 of bile salts solution using a 10cm3 measuring cylinder. Clean the measuring cylinder., Add 10cm3 of sodium carbonate solution using a 10cm3 measuring cylinder. Clean the measuring cylinder., Add 1o drops of phenolphthalein to the beaker. The mixture should be pink., Put the beaker into a water bath at 20oC and stir. Leave for 5 minutes for the contents of the beaker to reach the correct temperature., Add 5cm3 of the lipase solution and start the stopwatch., Stir the contents of the beaker until the mixture loses the pink colour. Stop the stopwatch., Record how long it takes until the pink colour disappears in your table and repeat twice more., Repeat the steps again at 30,40,50,60oC.

Decay required practical method

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