Make up a solution of known concentration (standard solution), Add ligand to standard solution and unknown to intensity colour, Make serial dilutions of standard solution to give 5 known concentrations across a range for the unknown, Measure the absorbance of light using a complementary colour filter/wavelength, Plot a calibration curve: absorbance vs concentration, Measure the absorbance of the solution with the unknown concentration, Use the calibration curve to determine the concentration of the solution.

Colorimetry method

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