Pre-Zygotic: Lions and Tigers live on different continents so never mate, Sea snakes and land snakes live in different habitats so never mate, The Galapagos has many Birds of Paradise species each one has its own unique mating dance, Fruit fly populations either feed on apple trees or pear trees and mate only with other flies that eat the same fruit, Spiny sea urchin sperm can only merge with the eggs of other spiny urchins and no other species, Once released into the ocean Yellowfin Tuna sperm can only merge with Yellowfin Tuna eggs, Short day orchids flower in Winter and Long day orchids flower in Winter, Post-Zygotic: Sheep and goats create embryos but these die before birth, Horses and donkeys can make offspring (mules) but they are infertile and cannot breed, Different cultivars of cotton plants can make hybrid offspring but they are weak and become sterile over a couple of generations,

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