What organism was used in this study? , Hermit Crabs, Parhyale Hawaiensis , Caenorhabditis elegans, Procambarus alleni, What biological process is the primary focus of this study? , neuronal migration , embryonic development , limb regeneration , wound healing , What fluorescent labeling strategy is primarily used for cell tracking?, Cytoplasmic GFP, Membrane-bound RFP, Nuclear-localized fluorescent protein, Dye injection at each time point, Why are nuclei labeled instead of entire cells?, nuclei are easier to segment and track , cytoplasm does not fluoresce well, nuclei move faster, cell membranes are too thick, What software platform is used for computer-assisted cell tracking?, CellProfiler , Elephant, TrackMate, What is the main advantage of computer-assisted tracking in this study?, It eliminates the need for microscopy , it perfectly tracks every cell, it replaces biological experiments , it allows long-term lineage reconstruction, What type of tracking strategy is emphasized when cell identity is determined at the end?, Forward tracking, random sampling , backtracking, population averaging , When is cell identity determined in this workflow?, before amputation , during live imaging, after live imaging using fixed tissue staining , it is never determined, Which technique is used to identify gene expression in fixed tissue?, western blot, HCR, PCR, flow cytometry , Why is post-hoc staining necessary in this study?, live markers do not show up well , cell identity cannot be fully determined during live imaging, antibodies do not work in crustaceans , imaging equipment is limited, Why do the authors say light-sheet microscopy would be preferable?, higher resolution only, it is faster and gentler on tissues , it is cheaper, it doesn't require mounting, What happens if the animal molts during the experiment?, nothing, cell tracking improves, regeneration speeds up , the experiment ends, Why is backtracking particularly well-suited for this regeneration model?, cells divide infrequently , cell identities are only known at the end of the experiment, cells do not migrate , imaging resolution improves over time, What feature of the mounting setup allows the animal to remain alive?, Continuous anesthesia, Complete immobilization, Only the regenerating leg is constrained, not the body, Nutrient feeding through the coverslip , What would be the worst time point to assign cell identity for lineage analysis?, immediately after amputation , mid-regeneration, after fixing and staining , randomly during imaging, What is the main conceptual advance of this paper?, a new regeneration gene , faster imaging hardware, eliminating the need for animal modelsEliminating the need for animal modelsIntegrating long-term live imaging with post-hoc cell identity and lineage reconstruction, integrating long-term live imaging with post-hoc cell identity and lineage reconstruction.

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