Column Chromatography: Stationary phase = powder in a glass column, Mobile phase = solvent (e.g. hexane or dichloromethane), One phase is polar while the other is non-polar., Use to separate large quantities of soluble substances, Retention time determined by affinity to each phase, Gas-Liquid Chromatography: Stationary phase = powder coated with a non-volatile liquid, Mobile phase = inert gas (e.g. Nitrogen), used to separate volatile liquids •Used to separate volatile liquids Used to separate volatile liquids , Generally attached to a mass spectrometer •Generally attached to a mass spectrometer Generally attached to a mass spectrometer , Retention time mainly determined by affinity to mobile phase, Thin Layer Chromatography: Stationary phase = plastic coated in cellulose, Mobile phase = solvent, Used to separate and identify soluble substances, Retention factor calculated, Developing agents used e.g. ninhydrin or UV light,

Chromatography

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