Origin of Replication - a specific sequence on the DNA molecule where the process of DNA replication begins, Replication Bubble - an unwound and open region of DNA where DNA replication occurs. Bubbles are created when the enzyme helicase separates the two strands of DNA so that they can be replicated., Leading Strand - The continuously synthesized strand, Lagging Strand - is synthesized in the opposite direction to the replication fork, and is replicated discontinuously. Small okazaki fragments. , Okazaki Fragment - Small synthesized fragments from the lagging strand, Primer - a short, single-stranded nucleic acid sequence that serves as a starting point for DNA synthesis, Primase - an enzyme that synthesizes RNA primers, which are short RNA sequences that serve as the starting point for DNA replication:, Polymerase - an enzyme that synthesizes long chains of polymers or nucleic acids., Pulse Chase - a two-phase technique used to examine cellular processes that take place over a period of time, Template Strand - the DNA strand that is used as a template to create messenger RNA (mRNA) during transcription, Daughter Strand - the newly synthesized strand of DNA that is copied via the addition of complementary nucleotides from one strand of pre-existing DNA during DNA replication., Ligase - an enzyme that joins two molecules by forming a new chemical bond, Gyrase - a bacterial enzyme that is responsible for the negative supercoiling of DNA, Helicase - enzymes that separate double-stranded nucleic acids into single strands,

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