1) DNA replication is considered semiconservative because... a) Both strands of the DNA are degraded b) Each new DNA molecule contains two new strands c) Each new DNA molecule contains one old and one new strand  d) Only one strand is copied during replication 2) Which enzyme is responsible for unwinding the DNA double helix at the replication fork? a) DNA polymerase III b) Helicase c) DNA ligase d) Primase 3) DNA polymerase III can begin DNA synthesis only after... a) Helicase loads onto the DNA b) An RNA primer provides a free 3' OH group c) DNA ligase forms phosphodiester bonds d) DNA polymerase I removes okazaki fragments 4) What is the purpose of DNA ligase in the replication fork? a) removes RNA primer and adds back complimentary DNA nucleotides b) Joins the free ends of DNA strands by forming phosphodiester bonds 5) Proofreading is when errors are fixed during ____ and ____.  a) DNA pol I and DNA pol III b) RNA pol I and RNA pol III c) DNA pol III and RNA pol I d) DNA pol I and RNA pol III 6) True or False: DNA replication is faster in eukaryotes and slower in bacteria a) True b) False 7) Which of the following is a requirement for DNA pol III? a) Needs a template/ parent strand b) needs a primer c) Needs NTP's d) Both a and b e) All of the above f) None of the above

Lecture 18: DNA Synthesis

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