What are the two phases of chromatography - The mobile phase and stationary phase, What is the mobile phase? - The solvent that moves through the paper, carrying different substances with it, What is the stationary phase? - The chromatography paper, Example of an aqueous solvent - Water - used as a solvent, Example of a non-aqueous solvent - Alcohol - used as a solvent, Pure substances produce how many spots? - Only one spot, Impure substances produce how many spots? - More than one spot, Two substances are likely to be the same if ... - they produce the same number of spots, and these match in colour and position, What is the formula for Rf value? - Distance moved by a spot / distance moved by the solvent front, If a carton reads "pure orange juice" does that mean it is a pure substance? - No - it contains different substances mixed together, a pure substance consists only of ... - only one element, What does a mixture consists of? - Two or more substances not chemically joined together, What does Rf stand for? - Retention factor, What is the affinity of the substance being tested??  - How well it 'sticks' to the chromatography paper, The most soluble substance being tested will ... - Moves furthest up the chromatography paper.,

Paper Chromatography

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