1) Who developed the first electron microscope? a) Robert Hooke b) Louis Pasteur c) Anton van Leeuwenhoek d) Ernst Ruska 2) What does a TEM use instead of lenses? a) Water b) Glass plates c) Magnets d) Light beams 3) Why do thick parts of a TEM specimen appear darker? a) Reflect more electrons b) Absorb more electrons c) Stain lighter d) Produce color 4) What is a major disadvantage of using TEM? a) Specimen must be dead b) Only live cells used c) Low magnification d) Produces color images 5) Which microscope creates a 3D image by scanning electrons? a) TEM b) STM c) SEM d) Light microscope 6) What is the main disadvantage of using SEM? a) Only nonliving cells observed b) Very low magnification c) Uses lenses instead of magnets d) Produces 2D images only 7) Which microscope can be used with live specimens? a) TEM b) SEM c) None of the above d) STM 8) How does STM form an image? a) Passing electrons through b) Detecting tunneling electrons c) Staining with metals d) Reflecting light 9) What type of image does STM produce? a) 2D internal cell image b) 3D surface image c) 3D atomic-level image d) Black-and-white shaded image 10) Which microscope is best for viewing internal structures? a) Light microscope b) SEM c) STM d) TEM

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