Origin of replication (OriC), Specific DNA region where bacterial DNA replication begins, DnaA, Binds to the origin and helps initiate opening of the DNA helix, Helicase (DnaB), Unwinds/separates the two DNA strands at the replication fork, DNA gyrase/topoisomerase, Relieves supercoiling and twisting produced as DNA is unwound, Single-strand binding proteins (SSBs), Bind separated DNA strads and prevent them from pairing back together, Primase, Synthesizes short RNA primers, RNA Primer, Provides the free 3' -OH needed for DNA polymerase to begin DNA synthesis, DNA polymerase III, Performs most new DNA synthesis by adding nucleotides to the 3' end, dNTPs, Building blocks used by DNA polymerase to synthesize DNA, Leading strand, Allows continuous DNA synthesis, Lagging strand, Synthesized discontinuously, Okazaki fragments, Short sections of newly synthesized DNA produced on the lagging strand, DNA polymerase I, Removes RNA primers and replaces them with DNA, DNA ligase, Seals remaining nicks to join adjacent DNA fragments into a continuous strand

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