DNA helicase, Catalyze unwind the double stranded DNA to become single stranded., Single-strand binding protein (SSB Protein), To stabilize and prevent the single stranded DNA from recoiling in helix until the strands are replicated., Topoisomerase, Helps relieve the strain caused by the unwinding of DNA double stranded., Primase, Synthesize RNA primer by adding RNA nucleotide that complementary to the DNA template., DNA polymerase III, Add free DNA nucleotides to the 3’ end of the short RNA primer., DNA polymerase I, Replaces the RNA primer by adding free DNA nucleotides producing new complete DNA strands., DNA ligase, Form phosphodiester bond to join Okazaki fragments together at lagging strand.

DNA Replication

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