Agglutination - Particulate antigen reacts with antibody, Precipitation - Soluble antigen reacts with antibody, complement fixation test - In the positive test : The available complement is fixed by Ag-Ab complex and no hemolysis of sheep RBCs occurs. So the test is positive for presence of antibodies., Indirect ELISA - The enzyme-labelled antibody binds the antibody of interest, that is bound to an immobilized antigen., Sandwich ELISA - The antigen of interest is captured between an immobilized antibody and the labelled antibody., Competitive ELISA - The sample antigen competes with an inhibitor antigen, binding to a limited amount of labelled antibody., Chemiluminescence immunoassay - The magnetic particles coated with antibodies or antigens as a solid phase to detect specific antigens or antibodies in patient samples by means of chemiluminescence signals., Direct Immunofluorescence - Using a primary antibody that is conjugated with fluorescent dye, Indirect Immunofluorescence - Using two antibodies for the staining: primary antibody that specifically binds to epitope and a matched secondary antibody conjugated with fluorescence dye, Single diffusion in two dimensions (Radial immunodiffusion) - for the quantitative measurement of antibody in sera, Immunochromatography - A combination of chromatography (separation of components of a sample based on differences in their movement through a sorbent) and immunochemical reactions., Radioimmunoassay (RIA) - the competition for a fixed amount of specific antibody between a known radiolabelled antigen and an unknown test antigen.,

Immunological techniques

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