Wash your hands and sterilise work space, Light a Bunsen burner to work under so updraft keeps airborne microbes away, Heat the wire loop until it is red hot to kill any bacteria living on it, Allow the loop to cool, Dip the loop into the bacteria you want to grow, Open the petri dish by tilting lid slightly, Streak the Bacteria across the agar jelly, Heat the used wire loop again to kill any bacteria, Seal the petri dish - but not all the way round, Wash your hands thoroughly, Incubate at 25 °C for 24-48 hours

Aseptic Technique

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