forward, The ____ primer binds to the minus strand., two, For each amplicon, there are ___ different types of primers., plus, The reverse primer binds to the ____ strand., three, Primers bind to the ___ prime ends of the target sequence., heat, Taq polymerase is used for PCR because it is ____ stable., Magnesium, The PCR reaction mixture contains _____ ions which are essential for the action of Taq polymerase., thermal, PCR is carried out by _____ cycling: heat, cool, heat, repeat., pathogen, To diagnose infection, primers must be designed that bind only to ______ sequences., between, Short tandem repeats are repeated sequences found _____ genes., complementary, If forward and reverse primers were ____ to each other, primer dimers would form (undesirable)., denature, Using Taq polymerase for extension ensures that it does not _____ when dsDNA is denatured to ssDNA., nucleoside, a dNTP is a ____ triphosphate., annealing, The 3 steps in PCR are denaturation, ____, extension., increase, In PCR, an ____ in temperature is used to accomplish what helicase usually does in vivo.

PCR

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