Get a TLC plate and rule a pencil line 1cm from the bottom., Put a small dot of each sample onto the pencil line. , Dry the dot and add another layer on top, drying the dot again., Label the dots you have put on the chromatography paper with pencil., Put the TLC plate into a beaker of solvent. The solvent must be below the dots., Add a lid to prevent solvent evaporation., Leave the TLC plate until the solvent front reaches around 1cm from the top., Remove TLC plate, mark the solvent front with a pencil and dry in a fume cupboard., Use a locating agent or UV light to show the positions of the spots, draw around spots with a pencil., Measure the distance from the base line to the solvent front and the middle of the dots., Calculate Rf values to identify the compounds..

TLC Chromatography method

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