Wash your hands or wear gloves, On the underside of the agar plate, use a marker pen to divide the plate into five equal sections., Using sterile forceps dip one paper disc into each of the antiseptic solutions., Place the disc carefully into the respective section on the unsealed agar plate containing the bacteria., Place the used forceps into the beaker of disinfectant., Seal the plate with sellotape ensuring there is a gap so that oxygen can enter and condensation can escape., Place the plate upside down and incubate for 3-4 days below 25oC, Place any used equipment in the beaker of disinfectant., Remove your agar place from the incubator and seal with extra tape, Measure the diameter of the clear zone around each disc using a ruler., Measure again at 90o to the first measurement and record in a table., Calculate the mean diameter..

Microbiology Required Practical Method

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