DNA is isolated from the nucleus of the animal cell, A plasmid is isolated from a bacterial cell, The gene that is needed is cut from the DNA by restriction enzymes, The plasmid is then cut open by restriction enzymes, The gene is then inserted into the plasmid, Ligase is used to glue the animal DNA into the bacterial plasmid, The plasmid is inserted into a bacteria cell which is encouraged to multiply millions of times, The plasmids are then inserted into your required cell..




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