Annealing - Process of H bond formation when complementary base pairs bind., DNA Primer - Short single stranded DNA. Enables DNA polymerase to bind to single-stranded DNA, Nucleotide - Organic base, sugar and phosphate; the monomer of DNA, Amplification - Exponential increase in the amount of DNA produced in PCR, Restriction endonuclease - Enzyme catalysing hydrolysis reaction, breaking sugar-phosphate backbone in the DNA double helix at a specific site, Taq Polymerase - DNA polymerase isolated from bacterium and used in PCR, PCR Cycle - The sequence of denaturation, annealing and extension, repeated in PCR, Thermus Aquaticus - Bacterium which lives in high temperatures such as hot springs; provides enzyme Taq polymerase, Gene Probe - Small length of single-stranded DNA used to locate a specific base sequence on a longer section of DNA, 95 degrees C FOR 60 s - Temperature and time required to separate DNA strands, Denaturation - Process of separation of DNA strands, 55 degrees C - Temperature required to allow primers to anneal, dNTP - Deoxyribonucleoside triphosphate, 72 degrees C - Temperature required for DNA polymerase to bind and begin working, Thermostable - Ability to maintain conformational shape and remain active at high temperatures, Kary Mullis - Scientist who invented PCR and received the Nobel Prize in 1993,

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